Response of Superovulation by Using FSH (Follicle Stimulating Hormone) and Sex Determination of Embryos Using PCR in Pesisir Cows of West Sumatra

Tinda Afriani, Fery Lismanto Sayful, Sumedi Sumedi, Dino Eka Putra, Endang Purwati

Abstract


This study  was conducted to determine the response of superovulation by giving 16 ml dosage  of FSH hormone to female Pesisir cattle.  The estrus schedule of 15 Pesisir cows was set by inserting CIDR (Controlled Internal Drug Release) into the vagina for 12 days. At day 10, all cattles were injected with FSH for three consequent days but with decreasing dosage. On the 3rd day, FSH injection was accompanied by PGFinjection and CIDR was removed. The detection of estrus was performed at  day 13. Natural mating was proceeded after the estrus signs visible. Collection of donor embryos was done on the 6th and 8th day after mating. The variables  measured were the response of superovulation, total number of corpus luteum, number of embryos and sex ratio. The results obtained were all  Pesisir cows responded to superovulation. The  average number of of corpus luteum and embryoes per cow were  5.93±3.17 and -----, respectively,  while the total of transferable embryoes were 90, with an average of 6.00 or 61.64%. The sexing of embryoes obtained in this study from 146 embryoes awere 76.03% males (111 embryoes) and 23.97% females (35 embryoes). Based on total of transferable embryoes, there were 51.37% male embryos and 11.28% of females embryos. The result of this study showed that the sex ratio of male embryos was higher than female embryos.




Keywords


Pesisir Cattle, response of superovulation, FSH, corpus luteum, total of embryos, PCR and sex ratio of male and female

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References


Abdullah, R.B., N.Y. Shariffah, M.R. Rahman' and W.E. Wan Khadijah. 2011. Effect of oFSH dosages in superovulation protocolon ovarian responses in goat. The 3rd International Conference on Sustainable Animal Agriculture for Developing Countries (SAADC2011) July 26-29, 2011 Nakhon Ratchasima, THAILAND.

Adrial. 2010. Potensi sapi Pesisir dan upaya pengembangannya di Sumatera Barat. J. Litbang Pertanian. 29 (2) : 66-72.

Afriani, T dan F. Lismanto. 2015. Penerapan Bioteknologi Reproduksi Dengan Manipulasi Embrio Sapi Pesisir Sebagai Plasma Nutfah Sumatera Barat. Laporan Hibah Penelitian LPPM Unand, Padang.

Ali, M.S., M.A.M.Y. Khandoker, M.A. Afroz and A.K.F.H. Bhuiyan. 2012. Ovarian Response to Different Dose Levels of Follicle Stimulating Hormone (FSH) in Different Genotypes of Bangladeshi Cattle. Asian-Aust. J. Anim. Sci. Vol. 25, No. 1: 52 - 58.

Alonso, R.V., J.A.A. Hellu, S.H. V. Perri, J.A. Visintin, and J.F Garcia. 2009. Factors affecting commercial sexing program and multiple genetic analysis perspectives of in vitro produced bovine embryos. Reprod. Fert. Dev 21: 227.

Alves, B.C.A., V.F.M. Hossepian De Lima, C. M. Teixeira and C.A. Moreira-Filho. 2003. Use of primers derived from a new sequence of the bovine Y chromosome for sexing Bos Taurus and Bos indicus embryos. Theriogenology, 56: 1415-1419.

Anwar, S. 2004. Kajian keragaman karakter eksternal dan DNA mikrosatelit sapi Pesisir Sumatera Barat. Disertasi. Pascasarjana IPB, Bogor.

Arum. W.P., T.N. Siregar dan J. Melia. 2013.Efek pemberian ekstrak hipofisa sapi terhadap respons superovulasi sapi Aceh. J. Med. Vet. Vol. 7 No. 2: 71-74.

Baril, G., P. Brebion and P. Chesne. 1993. Manuel de formation pour la transplantation embryonnaire chez la brebis et la chevre, Vol. 115. (Food and Agriculture Organisation of the United Nations; Rome, Italy.) [In French].

Bo, G.A, G.P. Adams, R.A. Pierson, R.J. Mapletoft. 1995. Exogenous control of follicular wave emergence in cattle. Theriogenology, 4: 31-40.

Bülbül, B., M. Kirbaş, Ş. Dursun and M. Köse. 2013. Superovulation in cows synchronized with two different progesterone+oestradiol protocols. Archiv Tierzucht 56 (15) 160-168.

Chen, A., X. Zi-rong and Y. Song-dong. 2007. Sexing goat embryos by PCR amplification of X- and Y-chromosome specific sequence of the Amelogenin gene. Asian-Aust. J. Anim. Sci. Vol. 20, No. 11: 1689-1693.

Choi, Y.H., D.L. Hartman and K. Hinrichs. 2009.Viability of equine blastocysts subjected to biopsy for preimplantation genetic diagnosis. Reprod, Fert and Dev. 21: 166-167.

Cushman, R.A., J.R. Wood, R.G. Slattery, D.T. Clopton, J. Smith, K.A. Beavers, W.E. Pohlmeier, J.W. Bergman, K.V. Moline, A.S. Cupp. 2010. Reproductive aging influences ovarian function in beef cows. Nebraska Beef Cattle Reports, 558.

Donaldson, L.E. 1985. Estimation of superovulation responses in donor cows. Vet. Record. 117: 33-34.

Ekici, H., N. Turan, B.H. Sontas, C.R. Helps, A. Senunver, H. Ylmaz. 2006. Sex determination of bovine embryos using polymerase chain reaction (PCR). Revue de Medicine Veterinaire, 157: 441-444.

Faber, D.C., J.A Molina, C.L. Ohlrichs, D.F. Vander Zwaag, L.B. Ferré. 2003. Cemmercialitation of animal biotechnology. Theriogenology, 59: 125-138.

FAO. 2005. Training Manual For Embryo Transfer In Cattle. Animal Reproduction Laboratory, Colorado State University, Fort Collins, CO 80523, USA.

Gonzalez-Bulnes, A., D. T.Barid, B. K. Campbell, M. J.Cocero, R.M.Garcia-Garcia, E.K.Inskeep, A.Lopez-Sebastian, A.S.McNeilly,J. Santiago-Moreno, C.J.H.Souza, and A.Veiga-Lopes. 2004. Multiple factors affecting the efficiency of multiple ovulation and embryo transfer in sheep and goats. Reprod. Fert and Dev. 16: 421-435.

Hafez, E.S.E. 1987. Reproduction and Breeding Techniques for Laboratory Animals. Lea and Febriger, Philadelphia.

Heinonen K, Shieferans T, Heinonen M. 1996. Oestrus synchronization in Ethiopian highland zebu cattle by means of intravaginal cloprostenol administration. Trop Anim Hlth Prod, 28:121 – 125.

Herren, R. 2000. The Science of Animal Agriculture. 2nd Ed. Delmar Thomson Learning, Albany.

Hirayama, H., A. Fujikawa, S. Kageyama, S. Moriyasu, K. Sawai, S. Onoe, and A. Minamihashi. 2008. Multiple genotyping in bovine pre-implantation embryo with whole genome amplification. Anim. Sci.79: 554-560.

Huhtinen M, Peippo J, Bredbacka P. 1997. Successful transfer of biopsied equine embryos. Theriogenology, 48: 361-367

Kanitz. W., S. Bhojwani, F. Becker and F. Schneider. 2006. Follicular Dynamic and Characteristics of Ovulations in Heifers after Ovsynch Treatment in The Last Third of the Estrous Cycle.

Lehloenya, K.C., J.P.C. Greyling, S. Grobler. 2008. Effect of season on the superovulatory response in Boer goat does. Small Ruminant Research, 78: 74-79.

Lopes, R.F.F., F. Forell, A.T.D. Oliveira, J.L. Rodrigues. 2001.Splitting and biopsy for bovine embryo sexing under field conditions. Theriogenology,, 56: 1383–1392.

Maidaswar. 2007. Efisiensi Superovulasi pada Sapi Melalui Sinkronisasi Gelombang Folikel dan Ovulasi. Tesis. Pascasarjana Institut Pertanian Bogor, Bogor.

Manna, L., G. Neglia, M. Marino, B. Gasparrini, R. Di Palo, and L. Zicarelli. 2003. Sex determination of buffalo embryos (Bubalus bubalis) by polymerase chain reaction. Zygote. 11: 17-22

Maret, D. 2001. Pengaruh dosis follicle stimulating hormone (FSH) dan body condition score (BCS) terhadap superovulasi sapi perah Fries Holstein (FH). Skripsi. Fakultas Peternakan, Institut Pertanian Bogor, Bogor.

Merton, J.S., A.P.W. de Roos, E. Mullaart, L. de Ruigh, L. Kaal, P.L.A.M. Vos and S.J. Dieleman. 2003. Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology, 59:651-674.

Muawanah. 2000. Superovulasi pada sapi perah Fries Holland (FH) dengan pemberian dosis FSH yang Berbeda. Skripsi. Fakultas Peternakan. Institut Pertanian Bogor, Bogor.

Nanda, M. 2012. Respon superovulasi sapi Pesisir dan sapi Simental. Tesis. Program Pascasarjana Universitas Andalas, Padang.

Nilchuen, P., S. Rattanatabtimtong and S. Chomcai. 2011.Superovulation with different doses of follicle stimulating hormone in Kamphaeng Saen beef cattle. Songklanakarin J. Sci. Technol. 33 (6), 679-683.

Peippo, J., S. Viitala, J. Virta, M. Raty, N. Tammiranta, T. Lamminen, J. Aro, H. Myllymaki, and J. Vilkki. 2007. Birth of correctly genotyped calves after multiplex marker detection from bovine embryo microblade biopsy. Molecular. Reprod. Dev. 74: 1373-1378.

Peura, T., J. M. Hyttinen, M. Turunen and J. Janne. 1991. A reliable sex determination assay for bovine preimplantation embryos using the polymerase chain reaction. Theriogenology. 35(3): 547- 555.

Prasetyo, D. 2012. Tingkat superovulasi pada beberapa bangsa sapi dengan sumber follicle stimulating hormone (FSH) yang berbeda. Skripsi. Fakultas Peternakan. Institut Pertanian Bogor.

Rahman, M. R., M. M. Rahman, W.E. Wan Khadijah and R.B. Abdullah. 2014. Comparison of superovulatory effect of equine chorionic gonadotrophin and follicle stimulating hormone on embryo production in crossbred (Boer × Katjang) goats. Pakistan J. Zool., Vol. 46(3): pp. 819-826.

Rajamahendran, R. 2002. Advanced Technology in Molecular Biology and Biotechnology of Farm Animals. Faculty of Agriculture The University of British Columbia, Vancouver, Canada.

Rensis, F.D. and R.J. Scaramuzzi. 2003.: Heat stress and seasonal effects on reproduction in the dairy cow – a review. Theriogenology 60, 1139–1151.

Rico, C., S. Fabre, C. Medigue, N. Di Clemente, F. Clement, M. Bontoux, J.L. Touze, M. Dupont, E. Briant, B. Remy, J.F. Beckers, D. Monniaux. 2009. Anti-mullerian hormone is an endocrine marker of ovarian gonadotropin-responsive follicles and can help to predict superovulatory responses in the cow. Biol. Reprod. 80, 50-59.

Rocha, E.H.R. 2005. Analysis of record of embryo production in Red Brahman cows. Thesis. Texas A&M University.

Saito, S. 1997. Manual on Embryo Transfer of Cattle. National Livestock Embryo Centre (BET Cipelang)-DGLS and Japan International Cooperation Agency (JICA).

Sato T., K. Nakada, Y. Uchiyama, Y. Kimura, N. Fujiwara, Y. Sato, M. Umeda and T. Furukawa .2005. The effect of pretreatment with different doses GnRH to synchronize follicular wave on superstimulation of follicular growth in dairy cattle. J of Reprod and Dev, 51(5): 573-578.

Seidel, G.E. and Elsden, R.P. 1985. Procedurs for Recovery, Bisection, Freezing and Transfer Bovine Embryos. Colorado (AS): Colorado State Univ.

Siregar, T.N., I.K. Siregar, T. Armansyah, Syafruddin, A. Sayuti dan Hamdani. 2013 Tampilan reproduksi kambing lokal hasil induksi superovulasi dengan ekstrak pituitary. Jur Vet. 14(1):75-79.

Siregar, T.N., T. Armansyah, A. Sayuti, Syafruddin. 2010. Tampilan reproduksi kambing betina lokal yang induksi berahinya dilakukan dengan sistem sinkronisasi singkat. Jur. Vet. Vol. 11 (1): 30-35.

Siregar, T.N., T. Armansyah, A. Sayuti, Syafruddin. 2010. Tampilan reproduksi kambing betina lokal yang induksi berahinya dilakukan dengan sistem sinkronisasi singkat. Jur. Vet. Vol. 11 (1): 30-35

Suradi, 2004. Kualitas embrio hasil inseminasi buatan (IB) sapi Limousin dan Simental dengan sapi Bali. Skripsi. Fakultas Peternakan. Institut Pertanian Bogor, Bogor.

Thibier, M. and M. Nibart. 1995. The sexing of bovine embryos in the field. Theriogenology, 43, 71–80.

Victorbuana. 2010. Peluang Usaha Ternak Sapi Potong. PeluangUsaha.web.id

Wright, R. 1987. Present Status of and Prospects for Embryo Transfer in the United States. In: Technical Meeting on Embryo Transfer and Animal Production. National Academy Press, Washington DC.

Yusuf, T.L. 1990. Pengaruh Prostaglandin F2α dan Gonadotropin terhadap aktivitas estrus dan superovulasi dalam rangkaian kegiatan transfer embrio pada sapi Fries Holand, Bali dan peranakan Ongole. Disertasi. IPB Bogor.

Yu, W., S. Li, J. Fang, X. Sun, L. Cui, J. Fu, Y. Bai, Y. Fang and B. Shangguan. 2007: Field studies on the effectiveness of the YCD embryo sexing technique in bovine. Reprod. Fert. and Dev, 19: 299-300.

Zeleny, R., A. Bernreuther, H. Schimmel and J. Pauwels. 2002. Evaluation of PCR-based beef sexing methods. J. Agric. Food. Chem. 50: 4169-4175.


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