Additional Freeze Drying Fig Fruit (Ficus Carica L) Filtrate into Tris Egg Yolk Extender and Its Effect on Sperm Membrane Integrity and  Acrosome of Kacang Buck

Lalu Ahmad Zaenuri; Lukman Lukman; Oscar Yanuarianto; I Wayan Lanus Sumadiasa; Rodiah Rodiah

doi:10.20884/1.jap.2017.19.3.647

Lalu Ahmad Zaenuri, Lukman Lukman, Oscar Yanuarianto, I Wayan Lanus Sumadiasa, Rodiah Rodiah

Abstract

A study was designed to determine Kacang buck sperm membrane integrity and acrosome reaction as to the effect of different concentration of freeze drying fig fruit filtrate in tris egg yolk based extender. This study used 5 proven fertility Kacang goats aged 3-4 years, maintained by Faculty of Animal Sciences, Mataram University, Indonesia. Semen was collected by artificial vagina at every five days. The collected semen was divided into four aliquots in accordance to the treatments extender such as Control (0 gr), T1 (0.02 gr), T2 (0.04 gr) and T3 (0.06 gr) freeze drying fig fruit filtrate in tris egg yolk based extender (gr/v), respectively. Plasma membrane integrity and intact acrosome after re-concentration and preserved at 5ºC were assessed visually at 0 and every 24 hours for 5 consecutive days. The statistical significance of the result was evaluated by a one way ANOVA for completely randomized design analysis of variance. Data were presented as Mean±SD. Results suggest that the mean percentages of sperm membrane integrity in T0, T1,T2 and T3 at 96 h post extended and preserve at 5ºC were 34.3±5.3, 40.6±4.7, 44.8±5.4 and 42.1±5,1, respectively. The mean percentages of sperm acrosome intact were 16.4±4.8, 18.5±1.9, 21.6±3.1 and 19.6±2.8, respectively. The results of the study suggested that additional 0.04gr freeze drying fig fruit filtrate into tris egg yolk based extender have a significant preservation effect on both spermatozoa membrane integrity and acrosome intact of kacang buck.

Keywords

Buck, Spermatozoa, Acrosome, membrane, fig fruit extract, freeze-drying

Full Text:

PDF

References

Ahmad Z. M Anzar, M Shahab, N Ahmad and SMH Andrabi. 2003. Sephadex and sephadex ion exchange filtration I mproves the quality and free ability of low-grade buffalo semen ejaculates. Theriogenology 59: 1189-202.

Anghel A, S Zamfirescu, C Dragomir, D Nadolu, S Elena and B Florica. 2010. The effects of antioxidants on the cytological parameters of cryopreserved buck semen. Romanian Biotechnological Letters. 15: 3. Supplement.

Ari UG and A Daşkin. 2010. Freezing of Washed Angora Goat Semen with Extenders Added Bull or Ram Seminal Plasma. Kafkas Univ Vet Fak Derg 16 (2): 233-37.

Bahmanpour S, T Talaei, Z Vojdani, MR Panjehshahin, IA Poostpasand and S Zareei. 2006. Ghaeminia Date palm pollen seems to cure male infertility by improving the quality of sperm parameters. However. further studies are needed to see its beneficial effects in man. Iranian Journal of Medical Sciences 31(4): 208-12.

Evans ACO and Lonergan C P. 2010. Effect of storage duration. storage temperature. and diluents on the viability and fertility of fresh ram sperm. Theriogenology 73: 541–49.

Garner DL and ESE Hafez. 2008. Spermatozoa and seminal plasma. In: Reproduction in Farm Animal. Hafez. B. And Hafez. E.S.E. 7th edn, Lippincott Williams and Wilkins. Awollers Kluwer Company. Philadelphi. 96-109.

Hafez ESE. 2008. Preservation and Cryopreservation of gametes and Embryos. In: Reprodustion in Farm Animals. Hafez. E.S.E. 7th edn, Lippincott Williams and Wilkins. Awollers Kluwer Company. Philadelphia: 431-42.

Hashida NH, RB Abdullah, MH Rajikin and M Mat Noor. 2005.Ultrastructural studies of fresh, frozen-thawed and acrosome- reacted goat sperm. Biomedical Research 16 (2): 119-23.

Mocé E and JK Graham. 2008. In vitroevaluation of sperm quality. Animimal Reproduction Sciences 105: 104-118.

Papa FO, GB Felício, CM Melo, MA Alvarenga, B DeVita, BR Avanzi and JA Dell’Aqua Jr. 2010. Effect of substituting soybean lecithin for egg yolk in an extender used for the cryopreservation of stallion semen. Animal Reproduction Science 121S: S171–S72.

Saraswat S, R Priyadharsini, SK Jindal, S Yadav, N Ramachandran, SD Kharche and AK Goel. 2012. Effect of Antioxidants Supplementation at RefrigerationTemperature on Sperm Motion Characteristics and Membran Integrity of Sirohi Buck Semen. Journal of Physiology Pharmmacy Advance. 2(1): 77-86.

Susilawati T. 2013. Spermatologi. Universitas Brawijaya Press. Malang, Indonesia.

Sumadiasa IWL, T Susilawati, G Ciptadi and N Isnaini. 2015. The potency of guava filtrate (Psidium guajava Linn) for preservation of Bali bull spermatozoa. Journal of Agriculture and Veterinary Science 8 (5): 51-57

Sacke RG. 1972. Semen quality tests and their relationship to fertility. NAAB procciding 4th Technology conference on animal Reproduction and Insemination 22-28.

Slover HT. 1983. Determination of tocopherols and sterols by capillary gas chromatography. Journal of the American Oil Chemists Society 60: 1524–28.

Talbot P, Shur B D and Myles D G. 2003. Cell adhesion and fertilization: steps in oocyte transport, sperm–zona pellucida interactions and sperm-egg fusion. Biology of Reproduction 68:1–9.

Hu JH, QW Li, YL Chen, ZL Jiang , YH Jia, LQ Wang and BB Ou. 2009. Effects of addition of vitamin B12 to the extender on post-thaw motility. acrosome morphology. and plasma membran integrity in bull semen. Turkish Journal of Veterinary and Animal Sciences. 33(5): 379-84.

Zaenuri LA, T Susilawati, SB Sumitro and S Wahyuningsih. 2013. The prospectus of local fig fruit (ficus glumerata Rob) extract to preserve goat sperm motility. Journal Kedokteran Hewan. 7(1): 26–28. (Article in Indonesian with an English Abstract).

Zaenuri LA, T Susilawati, SB Sumitro and S Wahyuningsih. 2014a. Effects of Additional Crude Extract of Fig Fruit (Ficus carica L) into Tris Egg Yolk Based Extender on Quality of Buck Semen. Journal of Biology, Agriculture and Healthcare 4 (9): 21-27.

Zaenuri LA, T Susilawati, SB Sumitro and S Wahyuningsih. 2014b. Preservation Effect of Crude Fig Fruit Filtrate (Ficus carica L) Added In to Tris Egg Yolk Based Extender on Capacitating. Acrosome and Fertility of Half Blood Boer Buck Spermatozoa. Journal of Agriculture and Veterinary Science 7 (5) Ver. II: 60-68.

Zamfirescu S, D Nadolu, V Ciupină and D Coprean. 2003. The biochemical and electronomicroscopic research of ram semen before and after freezing various extenders. National Congres of Biotechnology present and outlook in the third millennium. Bucharest-Roumania: 2 - 6.

DOI: http://dx.doi.org/10.20884/1.jap.2017.19.3.647