Efka Aris Rimbawanto, Lies Mira Yusiati, Endang Baliarti, Ristianto Utomo


Two plants as condensed tannin (CT) sources were supplemented to protein trash fish silage (TFS) to observe their effect on in vitro ruminal fermentation product, micobial protein synthesis, and   digestibility. CT supplementation on protein trash fish silage was on control proportion (0), under optimum level (2.0%), at optimum level (4.0%), and above optimum level (6.0%) of g TFS); of protein precipitation of bovine serum albumin (BSA) with CT from L. leucocephala; and under optimum level (1.5%), at optimum level (3.0%) and above optimum level (4.5% of g TFS), BSA protein precipitation with CT from C. calothyrsus. The effect on degradation in ruminal fluid and ruminal fluid followed by incubation in HCl-pepsin was evaluated using a modified two-step in vitro method. The CT level of L. leucocephala and C. calothyrsus was 4% and 6%, respectively with protein precipitation BSA was 26.25 and 31.77 g BSA/g CT, respectively. CT supplementation to trash fish silage, ruminal fermentation product (NH3-N, C2, C3, and total VFAs) and digestibility (DM and OM) decreased (P<0.01) and increased (P<0.01) total CP digestibility (in HCl-pepsin). The difference was attributed to CT source without affecting ruminal microbial protein synthesis. CT of L. leucocephala was better in increasing total protein digestibility (70%) than that of C. calothyrsus  (15%). It indicated that CT of C. calothyrsus was less effective in protected TSF protein degradation in rumen compared to that of L. leucocephala.

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AOAC (Asociation of Official Agricultural Chemists). 2005. Official Methods of Analysis. Agricultural Chemical; Contaminats; Drugs Vol. 1. Asociation of Official Agricultural Chemists, Inc. Virginia.

Bento MBL, HPS Makkar and T Acamovic. 2005. Effect of mimosa tannin and pectin on microbial protein synthesis and gas production during in vitro fermentation of 15N-labelled maize shoots. Animal Feed Science and Technology. 123–124:365–377.

Chavan UD, F Shahidi and M Naczk. 2001. Extraction of CTs from beach pea (Lathyrus maritimus L.) as affected by different solvents. Food Chemistry. 75:509–512.

Cortés JE, B Moreno, ML Pabón, P Avila, M Kreuzer, HD Hess and JE Carulla. 2009. Effects of purified CTs extracted from Calliandra, Flemingia and Leucaena on ruminal and postruminal degradation of soybean meal as estimated in vitro. Animal Feed Science and Technology. 151:194–204.

Getachew G, W Pittroff, DH Putnam, A Dandekar, S Goyal and E.J. DePeters. 2008a. The influence of addition of gallic acid, tannic acid, or quebracho tannins to alfalfa hay on in vitro rumen fermentation and microbial protein synthesis. Animal Feed Science and Technology. 140: 444–461.

Getachew G, W Pittroff, EJ DePeters, DH Putnam, A Dandekar and S Goyal. 2008b. Influence of tannic acid application on alfalfa hay: in vitro rumen fermentation, serum metabolites and nitrogen balance in sheep. Animal. 2(3):381–390.

Hess HD, M.L. Mera, TT Tiemann, CE Lascano and M Kreuzer. 2008. In vitro assessment of the suitabilaity of replacing the low-tannin legume Vigna unguiculata with the tanniniferous legumes Leucaena leucocephala, Flemingia macrophylla or Calliandra calothyrsus in a tropical grass diet. Animal Feed Science and Technology. 147:105–115.

Huang XD, JB Liang, HY Tan, R Yahya, B Khamseekhiew, and YW Ho. 2010. Molecular weight and protein binding affinity of Leucaena CTs and their effects on in vitro fermentation parameters. Animal Feed Science and Technology. 159:81–87.

IBM SPSS Statistics. 2013. Version 22, IBM Corporation. USA.

Jayanegara A, E Wina, CR Soliva, S Marquardt, M Kreuzer and F Leiber. 2011. Dependence of forage quality and methanogenic potential of tropical plants on their fenolic fractions as determined by principal component analysis. Animal Feed Science and Technology. 163: 231–243.

Jones WT, RB Broadhurst and JW Lyttleton. 1976. The CTs of pasture legume species. Phytochemistry. 15:1407–1409.

Makkar HPS, P Siddhuraju and K Becker. 2007. Plant Secondary Metabolites. Humana Press Inc. New Jersey. Pp 67-81.

Makkar HPS. 2003. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research. 49:241–256.

Markom M, M Hasan, WRW Daud, H Singh and JM Jahim. 2007. Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods. Separation and Purification Technology. 52:487–496.

McSweeney CS, B Palmer, DM McNeill and DO Krause. 2001. Microbial interaction with tannins: nutritional consequences for ruminants. Animal Feed Science and Technology. 91:83-93.

Min BR, TN Barry, GT Attwood and WC McNabb. 2003. The effect of CTs on the nutrition and health of ruminants fed fresh temperate forages: a review. Animal Feed Science and Technology. 106:3–19.

Mohammadabadi T and M Chaji, 2012. The Influence of the plant tannins on in vitro ruminal degradation and improving nutritive value of sunflower meal in ruminants. Pakistan Veterinary Journal. 32(2):225-228.

Mueller-Harvey I. 2006. Unravelling the conundrum of tannins in animal nutrition and health: a review. Journal of the Science of Food and Agriculture 86: 2010–2037.

Nsahlai IV, FN Fon and NAD Basha. 2011. The effect of tannin with and without polyethylene glycol on in vitro gas production and microbial enzyme activity. South African Journal of Animal Science. 41(4):337-344.

Plummer DT. 1987. An Introduction to Practical Biochemistry. McGraw Hill Ltd. Bombay, New Delhi. 352 pages.

Santoso, B., E. W. Saragih, and B.T. Hariadi. 2013. Effect of water extract of plants containing tannin on in vitro methanogenesis and fermentation characteristics of the grass Pennisetum purpureophoides. Journal of the Indonesian Tropical Animal Agriculture. 38 (1):47-54.

Sasongko WT, LM Yusiati, Z Bachruddin dan Mugiono. 2010. Optimalisasi pengikatan tanin daun nangka dengan protein bovine serum albumin. Buletin Peternakan. 34(3):154-158.

Satter LD and LL Slyter. 1974. Effect of ammonia concentration of rumen microbial protein production in vitro. British Journal of Nutrition. 32:199–208.

Shahidi F and M Naczk. 2004. Phenolics in Food and Nutraceuticals. CRC Press. Washington. 566 pages.

Sivakumaran S, AL Molan, LP Meagher, B Kolb, L. Foo, GA Lane, GA Attwood and K Fraser. 2004. Michael Tavendale a Variation in antimicrobial action of proanthocyanidins from Dorycnium rectum against rumen bacteria. Phytochemistry. 65:2485–2497.

Smith AH, E Zoetendal and RI Mackie. 2005. Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microbial Ecolology. 50:197–205.

Stürm CD, TT Tiemann, CE Lascano, M Kreuzer and HD Hess. 2007. Nutrient composition and in vitro ruminal fermentation of tropical legume mixtures with contrasting tannin contents. Animal Feed Science and Technology. 138: 29–46.

Susanti S dan E Marhaeniyanto. 2014. Kadar saponin daun tanaman yang berpotensi menekan gas metan secara in vitro. Buana Sains. 14(1):29-38.

Tejirian A and F Xu. 2011. Inhibition of enzymatic cellulolysis by fenolic compounds. Enzyme and Microbial Technology. 48:239-247.

Tiemann TT, CE Lascano, HR Wettstein, AC Mayer, M Kreuzer and HD Hess. 2008a. Effect of the tropical tannin-rich shrub legumes Calliandra calothyrsus and Flemingia macrophylla on methane emission and nitrogen and energy balance in growing lambs. Animal. 2(5):790–799.

Tiemann TT, LH Franco, G Ramírez, M Kreuzer, CE Lascano and HD Hess. 2010. Influence of cultivation site and fertilisation on the properties of CTs and in vitro ruminal nutrient degradation of Calliandra calothyrsus, Flemingia macrophylla and Leucaena leucocephala. Animal Feed Science and Technology. 157:30–40.

Tiemann TT, P Avila, G Ramírez, CE Lascano, M Kreuzer and HD Hess. 2008b. In vitro ruminal fermentation of tanniniferous tropical plants: plant-specific tannin effects and counteracting efficiency of PEG. Animal Feed Science and Technology. 146:222–241.

Tilley JMA and RA Terry. 1963. A two-stage technique for the in vitro digestion of forage crops. Journal of the British Grassland Society. 18(2):104.

Utomo, R. 1997. Kemungkinan penggunaan daun kaliandra sebagai pengganti daun lamtoro untuk pakan kambing. Bulletin Peternakan. 21(1):56-62.

Utomo, R. 2010. Modifikasi metode penetapan kecernaan in vitro bahan kering atau bahan organik. Bulletin Sintesis. ISSN: 0853-9812. 14(1):1 - 11.

Wheatherburn, M.W. 1967. Phenol-hypoclorite reaction for determination of ammonia. Analytical Chemmistry. 39: 971-974.

Wischer G., J. Boguhn, H. Steingass, M. Schollenberger, and M. Rodehutscord. 2013. Effects of different tannin-rich extracts and rapeseed tannin monomers on methane formation and microbial protein synthesis in vitro. Animal. 7(11): 1796-805.

Yusiati, L.M., Z. Bachrudin, C. Hanim, dan W.K. Ratriningtyas, 2007. Pengaruh Daun Ketepeng Cina Terhadap Produksi Gas Methan, Asam Lemak Volatile Dan Mikrobia Pada Fermentasi Substrat Dengan Rasio Hijauan Dan Konsentrat Yang Berbeda Menggunakan Mikrobia Rumen Secara In Vitro. Proseding Seminar Nasional Dies Natalis ke-38 Fakultas Peternakan UGM. Yogjakarta. ISBN: 978-979-1215-10-7. Hal. 102-107.

DOI: http://dx.doi.org/10.20884/1.anprod.2015.17.2.505

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