Polymorphism of Myostatin Gene (MSTN) Coding Region in Batur Sheep

Hassan Ishag Hassan Haren; Prayitno Prayitno; Dattadewi Purwantini; Mas Yedi Sumaryadi

doi:10.20884/1.jap.2019.21.1.780

Hassan Ishag Hassan Haren, Prayitno Prayitno, Dattadewi Purwantini, Mas Yedi Sumaryadi

Abstract

The aim of present study was to investigate myostatin gene polymorphism and its association with weaning weight and body weight in Batur sheep, 30 heads of Batur all lambs were fed by concentrated feed. Body weight measured monthly after weaning to the six months of age. DNA Extraction used 200 ul of whole blood samples. To amplify exon 3 region of MSTN gene a specific primer designed using the Primer3 software. The 25 Î¼l volume contained 25 ng of genomic DNA, 12.5 Î¼l 2x Reaction mix of each primer. The cycling protocol was 5 minutes at 95Â°C as initial denaturation, 35 cycles of denaturing at 94Â°C for 45 seconds, annealing at 73.9 for 45 seconds, extending at 72Â°C for 40 s, with final extension at 72Â°C for 10 minutes. Eleven polymorphic sites were observed in the in 3rd exon region transversions at c.*121 G instead of A, del-T at c.*129, one individual at c.*139 and one individual at c.*158 positions however, one individual sequence disrupted reading frame in whole MSTN sequenced, also weaning weight and 6-month weight of Batur lambs were 21.13Â±5.70 and 31.64Â±7.06 to the homozygous whilst 19.99Â±4.33 and 30.50Â±5.44 for heterozygote respectively but there is no significe differences seen above all the studied parameters. Homozygous AA lambs had fairly low weaning weight and body weight compared to the heterozygous AB lambs but might have a higher carcass weight, further investigation is needed into the interaction of MSTN with other genes involved in muscle growth.

Keywords

Polymorphism, Association, MSTN gene and Batur sheep

Full Text:

PDF

References

Boman Inger A., Gunnar Klemetsdal, Ola Nafstad, Thor Blichfeldt, Dag I. VÃ¥ge (2010). Impact of two myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian White Sheep (Ovis aries) Genetics Selection Evolution 201.

Boman, I.A., VÃ¥ge, D.I., 2009. An insertion in the coding region of the myostatin (MSTN) gene affects carcass conformation and fatness in the Norwegian SpÃ¦lsau (Ovis aries). BMC Res. Notes 2, 98â€“102.

Clop A., Marcq F., Takeda H., Pirottin D., Tordoir X., Bibe B., Bouix J., Caiment F., Elsen J. M., Eychenne F., Larzul C., Laville E., Meish F., Milenkovic D., Tobin J., Charlier C., Georges M. (2006): A mutation creating a potential illegitimate microRNA target site in the myostatingene affects muscularity in sheep. Nature Genetics, 38, 813â€“818.

Dunner, S., Miranda, M.E., Amigues, Y., CanÃ³n, Ëœ J., Georges, M., Hanset, R., Williams, J.L., MÃ©nissier, F., 2003. Haplotype diversity of the myostatin gene among beef cattle breeds. Genet. Sel. Evol. 35, 103â€“118.

Grobet, L., Martin, L.J.R., Poncelet, D., Pirottin, D., Brouwers, B., Riquet, J., Schoeberlein, A., Dunner, S., Menissier, F., Massabanda, J., Fries, R., Hanset, R., Georges, M., 1997. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat. Genet. 17, 71â€“74.

Hickford, J.G.H., Forrest, R.H., Zhou, H., Fang, Q., Han, J., Frampton, C.M., Horrell, A.L., 2010. Polymorphisms in the ovine myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand Romney sheep. Anim. Genet. 41, 64â€“72.

Johnson, P. L., McEwan, J. C., Dodds, K. G., Purchas, R. W., & Blair, H. T. (2005). A directed search in the region of GDF8 for quantitative trait loci affecting carcass traits in Texel sheep. Journal of Animal Science, 83, 1988-2000.

Johnson, P.L., Dodds, K.G., Bain, W.E., Greer, G.J., McLean, N.J., McLaren, R.J., Galloway, S.M., van Stijn, T.C., McEwan, J.C., 2009. Investigations into the GDF8 g+6273G-A polymorphism in New Zealand Texel sheep. J. Anim. Sci. 87, 1856â€“1864.

Kijas, J.W., McCulloch, R., Edwards, J.E., Oddy, V.H., Lee, S.H., van der Werf, J., 2007. Evidence for multiple alleles effecting muscling and fatness at the Ovine GDF8 locus. BMC Genet. 8, 80â€“90.

Lee, S.J., McPherron, A.C., 2001. Regulation of myostatin activity and muscle growth. Proc. Natl. Acad. Sci. U.S.A. 98, 9306â€“9311.

Marcq, F., Larzul, C., Marot, V., Bouix, J., Eychenne, F., Laville, E., BibÃ©, B., Leroy, P.L., Georges, M., & Elsen, j. M. (2002). Preliminary results of a whole-genome scan targeting QTL for carcass traits in a Texel X Romanov intercross. Proceedings of the 7th World Congress on Applied Livestock Production (Montpellier, France), 12-14.

McPherron, A.C., Lawler, A.M., Lee, S.J., 1997. Regulation of skeletal muscle mass in mice by a new TGF- superfamily member. Nature 387, 83â€“90.

Mendias, C.L., Bakhurin, K.I., Faulkner, J.A., 2008. Tendons of myostatindeficient mice are small, brittle, and hypocellular. Proc. Natl. Acad. Sci. U.S.A. 105, 388â€“393.

Mosher, D.S., Quignon, P., Bustamante, C.D., Sutter, N.B., Mellersh, C.S., Parker, H.G., Ostrander, E.A., 2007. A mutation in the myostatin gene increase muscle mass and enhances racing performance in heterozygote dogs. PLoS Genet. 3, 779â€“786.

Parker, M.H., Seale, P. and Rudnicki, M.A. 2003. Looking back to the embryo: defining transcriptional networks in adult myogenesis. Nature Reviews Genetics 4:497-507.

Rehfeldt, C., Fiedler, I., Dietl, G. and Ender, K. 2000, Myogenesis and postnatal skeletal muscle cell growth as influenced by selection. Livestock Production Science 66:177-188.

Smith SJ, Cases S, Jensen DR, Chen HC, Sande E (2000) Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking DGAT. Nat Genet 25:87â€“90.

Stinckens, A., Luyten, T., Bijttebier, J., Van den Maagdenberg, K., Dieltiens, D., Janssens, S., De Smet, S., Georges, M., Buys, N., 2008. Characterization of the complete porcine MSTN gene and expression levels in pig breeds differing in muscularity. Anim. Genet. 39, 586â€“596.

Walling, G. A., Visscher, P. M., Simm, G., & Bishop, S. C. (2001). Confirmed linkage for QTLs affecting muscling in Texel sheep on chromosomes 2 and 18. Proceedings of 52nd Animal Meeting of the European Association for Animal Production, Budapest, Hungary, 26th-29th August 2001, Paper G5.6.

Zhou, H., Hickford, J. G. H., & Fang, Q. (2008). Variation in the coding region of the myostatin (GDF8) gene in sheep. Molecular and Cellular Probes, 22, 67-68.

DOI: http://dx.doi.org/10.20884/1.jap.2019.21.1.780