In vitro anthelmintic activity of the extract of coffee husk fermented with Pleurotus ostreatus for Ascaridia galli

Irma Badarina, Heru Dwi Putranto, Endang Sulistyowati

Abstract


The objective of  this research was to evaluate the anthelmintic  efficacy of the ethanolic and aqueous extract of coffee husk fermented by Pleurotus ostreatus against Ascaridia galli.  In vitro study of anthelmintic activity was conducted by counting the number of paralyzed worm body of Ascaridia galli during 24 hours in petri dish containing different levels of ethanolic and aqueous extract of fermented coffee husk, namely 0%, 1%, 2%, 3% and 4% (w/v) and compared to piperazine 0,5% (w/v) and NaCl 0,9%.   The powdered  samples of fermented coffee husk  were macerated in the 30% ethanol solution  with the ratio samples to solution = 1:3.   In aqueous extract, samples to solution ratio was 1 : 7.  The extract of fermented coffee husk possess phytochemical compunds which responsible for anthelmintic activity.   The anthelmintic activity of ethanolic extract was better than aqueous extract.  Fermented coffee husk ethanolic extract exhibited mortality of A.galli at concentration 2% while showed highest mortality at concentration 4%.  Fermented coffee husk ethanolic extract have the potential to aid in the control of gastro intestinal parasites in animal



Keywords


fermented coffee husk, extract, anthelmintic, A.galli

Full Text:

PDF

References


Akhtar M.S, Iqbal Z., Khan M.N. and Lateef M. Anthelmintic activity of medicinal plants with particular reference to their use in animals in the Indo-Pakistan subcontinent. Small Ruminant Research 2000; 38: 99-107.

Badarina, I., D. Evvyernie , T. Toharmat , E.N. Herliyana and L.K. Darusman. 2014. The use of coffee husk fermented with Pleurotus ostreatus as feed supplement improved haematological properties in Etawah dairy goat suffered from subclinical mastitis. The Proceeding of The 2nd Asian-Australasian Dairy Goat Conference. Faculty of Animal Science, Bogor Agricultural University, Bogor, Indonesia. April 25-27th, 2014, Pp:155-157

Bressani, R. 1979. Antiphysiological factors in coffee pulp. Coffee Pulp:Composition, Technology and Utilization. IDRC Pub. 108, 1979.

C. Marcos-Atxutegi, B. Gandolfi, T. Arangüena, R. Sepúlveda, M. Arévalo, F. Simón. 2009. Antibody and inflammatory responses in laying hens with experimental primary infections of Ascaridia galli. Veterinary Parasitology. Vol 161, issues 1-2, pages 69-75

Fan, L., A. Pandey, R. Mohan, & C. R. Soccol. 2000. Use of various coffee industry residues for the production of Pleurotus ostreatus in solid state fermentation. Acta Biotechnol. 20(1): 41-52.

Ganeshpurkar, A., S.S. Bhadoriya, P. Pardhi, and G. Rai. 2012. Investigation of anthelmintic potential of oyster mushroom Pleurotus florida. Indian J. Pharmacol. 44(4): 539–540.

Gee, J. M. and Johnson, I. T. (1988). Interaction between haemolytic saponins, bile salts and small intestinal mucosa in rat. Journal of Nutrition, 118: pp 1391–1397

Harborne, J. B. 1989. General procedures and measurement of total phenolics. In J. B.Harborne (Ed.). Methods in plant biochemistry: Volume 1 Plant Phenolics (pp. 1–28). London:Academic Press.

Herliyana, E. N., D. Nandika, Achmad, L. I. Sudirman and A. B. Witarto. 2008. Biodegradation of sengon-wood sawdust substrate by Pleurotus group fungi from Bogor. Journal of Trop. Wood Sci. and Technol. 6:75-84.

Min B. R. and S. P. Hart. 2003. Tannins for suppression of internal parasites. J. Anim. Sci. 81(E. Suppl. 2):E102–E109.

Patel, Y., R. Naraian and V.K. Singh. 2012. Medicinal properties of Pleurotus species (Oyster mushroom): A review. World J. of Fungal and Plant Biol. 3(1):01-12.

Pathak, A.K., N. Dutta, P. S. Banerjee, A. K. Pattanaik and K. Sharma. 2013. Influence of dietary supplementation of condensed tannins through leaf meal mixture on intake, nutrient utilization and performance of Haemonchus contortus infected sheep. Asian Australas. J. Anim. Sci. 26 (10): 1446-1458.

Sissouma, S., M. Ouattara, M.W. Koné, H.E. Menan, A. Adjou and L. Ouattara. 2011. Synthesis and in vitro nematicidal activity of new chalcones vectorised byimidazopyridine. Research paper. African J. of Pharmacy and Pharmacology. 5(18): 2086-2093.

Suharti, S., K.G. Wiryawan, R. Tiuria, Y. Ridwan, S. Fitriana and N. Sumarni. 2010. Effectiveness of Jatropha curcass Linn leaves as an anthelmintic for Ascaridia galli and its effect on native chicken performance. Med. Pet. 33(2): 108-114

Wolstenholme, A.J, I. Fairweather, R. Prichard, G. von Samson- Himmelstjerna and N.C. Sangster. 2004. Drug resistance in veterinary helminthes. Trends in Parasitology. 20: 469-476

Wong, K.H., C.K.M. Lai and P.C.K. Cheung. 2011. Immunomodulatory activities of mushroom sclerotial polysaccharides. Food Hydrocolloids. 25:150-158.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Powered by OJS | Design by ThemeOJS