Although domestic cavies are widely used in sub-Saharan Africa as a source of meat and income, there are only a few studies of their population structure and genetic relatedness. This seminal study was designed with the main objective to assess the genetic diversity and determine the population structure of cavy populations from Cameroon to guide the development of a cavy improvement program. Sixteen microsatellite markers were used to genotype 109 individuals from five cavy populations (Wouri, Moungo and Nkongsamba in the Littoral region, and Mémé and Fako in the Southwest region of Cameroon). Twelve markers worked in the five populations with a total of 17 alleles identified, with a range of 2.9 to 4.0 alleles per locus. Observed heterozygosity (from 0.022 to 0.277) among populations was lower than expected heterozygosity (from 0.42 to 0.54). Inbreeding rates between individuals of the populations and between individuals in each population were 59.3% and 57.2%, respectively, against a moderate differentiation rate of 4.9%. All the tested loci deviated from Hardy-Weinberg equilibrium, except for locus 3. Genetic distances between populations were small (from 0.008 to 0.277), with a high rate of variability among individuals within each population (54.4%). Three distinct genetic groups were structured. This study has shown that microsatellites are useful for the genetic characterization of cavy populations in Cameroon and that the populations investigated have sufficient genetic diversity that can be used to be deployed as a basis for weight, prolificacy and disease resistance improvement. The genetic of diversity in Southern Cameroon is wide and constitute an opportunity for cavy breeding program.
Ayagirwe, RBB, Meutchieye, F, Wikondi, J, Poutougnigni, YM, Niba, AT and Manjeli, Y. 2015. Variabilité phénotypique dans les populations de cobayes domestiques (Cavia porcellus) du Cameroun, Bull. Anim. Hlth. Prod. Afr. AnGR Special Edition, 43 - 50
Ayagirwe, RBB, 2014. Diversité génétique et structure des populations de cobayes dans la zone agro-écologique monomodale du Sud Cameroun. MSc thesis, University of Dschang, Dschang, Cameroon.
Burgos-Paz W, Cerón-Muñoz M, Solarte-Portilla C. 2011. Genetic diversity and population structure of the Guinea pig (Cavia porcellus, Rodentia, caviidae) in Colombia, Gen. Mol. Biol. 34, 711-718.
Chauca, Z L. 1997. Producción de cuyes (Cavia porcellus). Food and Agriculture Organization (FAO), Rome, Italy. Website last accessed 2015 Aug. 11: http://www.fao.org/docrep/w6562s/w6562s00.htm.
Crow, JF, Kimura, M. 1970. An introduction to population genetics theory. Harper and Row, New York, Evanston and London.
Evanno, G, Regnaut, S, Goudet, J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol. 14, 2611-2620.
Excoffier, L, Laval, G, Schneider, S. 2005. Arlequin 3.0: An integrated software package for population genetics data analysis. Evol. Bioinf. Online 1, 47-50. Website last accessed 2015 Aug. 11: http://cmpg.unibe.ch/software/arlequin3.
FAO. 2008. Rapport national sur l'état des ressources phytogénétiques pour l'alimentation et l'agriculture: Cameroun. Institute de Recherche Agricole pour le Developpement (IRAD), Yaoundé, Cameroon. Website last accessed 2015 Aug. 11: http://www.fao.org/docrep/013/i1500e/Cameroun.pdf.
Felsenstein, J. 2005. Phylip (Phylogeny Inference Package) version 3.6. Department of Genome Sciences, University of Washington, Seattle, USA. Website last accessed 2015 Aug. 11: http://evolution.genetics.washington.edu/phylip.html.
Foulley, JL, Ollivier, L. 2006. Diversité génétique et richesse allélique: concepts et application à des races bovines. Renc. Rech. Ruminants 13, 227-230.
Granevitze, Z, Hillel, J, Chen, GH, Cuc, NT, Feldman, M, Eding, H, Weigend, S. 2007. Genetic diversity within chicken populations from different continents and management histories. Anim. Genet. 38, 576-583.
Hardouin, J. 1995. Minilivestock: from gathering to controlled production. Biodiv. Conserv. 4, 220-232.
Hubisz, MJ, Falush, D, Stephens, M and Pritchard, JK. 2009. Inferring weak population structure with the assistance of sample group information. Mol. Ecol. Resour. 9, 1322-1332.
Kanitz, R, Trillmich, F, Bonatto, SL. 2009. Characterization of new microsatellite loci for the South-American rodents Cavia aperea and Cavia magna. Conserv. Genet. Resour. 1, 47-50.
Kosaki, T, Juo, A SR. 1989. Multivariate approach to grouping soils in small fields. 1. Extraction of factors causing soil variation by Principal Component Analysis. Soil Sci. Plant Nut. 35, 469-477.
Kouakou, NDV, Speybroeck, N, Assidjo, EN, Grongnet, JF, Thys, E. 2011. Typifying guinea pig (Cavia porcellus) farmers in urban and peri-urban areas in central and southern Côte d’Ivoire. Outlook on Agric. 40, 323-328.
Kouakou, KP, Skilton, R, Djikeng, A, Fantodji, A, Gourene, B, Aoussi, SC. 2015. Genetic diversity and population structure of cavy (Cavia porcellus L) in three agro ecological zones of Côte d'Ivoire. IJAAR. 6, 27-35.
Kumar, S, Gupta, J, Kumar, N, Dikshit, K, Navani, N, Jain, P, Nagarajan, M. 2006. Genetic variation and relationships among eight Indian riverine buffalo breeds. Mol. Ecol. 15, 593-600.
Lammers, PJ, Carlson, SL, Zdorkowski, GA, Honeyman, MS. 2009. Reducing food insecurity in developing countries through meat production: the potential of the guinea pig (Cavia porcellus). Renew. Agric. Food Syst. 24, 155-162.
Liu, K, Muse, SV. 2005. Power Marker: Integrates analysis environment for genetic marker data. Bioinf. 21, 2128-2129.
Manjeli, Y, Tchoumboue, J, Njwe, RM, Teguia, A. 1998. Guinea-pig productivity under traditional management. Trop. Anim. Health Prod. 30, 115-122.
Matthiesen, T, Nyamete, F, Msuya, JM, Maass, BL. 2011. Importance of guinea pig husbandry for the livelihood of rural people in Tanzania: a case study in Iringa Region. Presented at Development at the Margin, Tropentag, 5-7 Oct. 2011, University of Bonn, Germany. Book of Abstracts, p. 342. Website accessed 2015 Aug 10: http://www.tropentag.de/2011/abstracts/links/Matthiesen_llDdf2DY.pdf.
Mohamed, OA, Farhat, BS, Bedhiaf, S, Djemali, M. 2010. Analyse moléculaire de la diversité génétique des dromadaires (Camelus dromedarius) en Tunisie. Biotechnol. Agron. Soc. Environ. 14, 399-408.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583-590.
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