Cytological Characteristics of Mucose Cell and Vaginal Temperature and pH During Estrous Cycle in Local Sheep

Siti Darodjah Rasad, Rangga Setiawan


Aim of this study was to examine the characteristics cytology of mucous cell-,temperature- and pH vagina during estrous cycle in local sheep.  31local sheep were synchronized with vaginal sponge consist of 20  mg  progesterone hormone before carried out observations of cytology of cells from the vaginal mucose through vaginal swabs, temperature and pH of the vagina.  Vaginal swabs were collected daily at 7 am for a weeks.Vaginal temperature and pH measurement is carried out twice a day, at 07.00 am and 15.00 pm for a weeks after vaginal swabs. Smears of the swab were then prepared on glass slide and they were stained with Giemsa.  Vaginal epithelial cells; Parabasal, intermediate and superficial cells were counted and their percentages during pro-estrous, estrous and di-estrous were determined. Di-estrous was characterized by the absent of superficial cells in the epithelial vagina. Pro-estrous was characterized by the increasing progressively of intermediate/superficial cells in epithelial vagina, whereas estrous was characterized by the presence of superficial/cornification cells in most epithelial vagina. Based on the dominance of superficial cell, the number of sheep identified as estrous is highest on third day, with 52%.  Observation on vaginal temperature also resulting that the highest temperature values obtained on the third day of 39,08±0.28°C.  It could be effected of the vaginal pH during the observation. Underthe influence ofestrogen, the epithelial vaginalcellssynthesizeand accumulateglycogenin large quantitiesdepositedin the lumen ofvagina. Vaginal bacteriametabolizethe glycogenformlactic acid, which causesvaginal pHis low.The pH conditions prevent from pathogenic microorganisms and fungi. Increased estrogenal so cause cell proliferation through the thickening of the epithelium lining of the vagina so that the cells differentiate.Increasing of glycogenin the superficial cells, and  ceratin cells found in the cytoplasm of cells, as well as acidic pH in the lumen vagina causing changes in the chemical composition of each cell (Zaid, 2011).It can be concluded that during estrous phase, cytology of  vaginal mucose cell is dominated by superficial cell and vaginal temperature is increasing as well as pH of vagina.


cytology, vaginal temperature, vaginal pH, estrous cycle, sheep

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Blaszczyk B, J Udala, and D Gaczarzewicz. 2004. Changes in estradiol, progesterone, melatonin, prolactin and thyroxin concentrations in blood plasma of goats following induced estrous in and outside the natural breeding season. Small Ruminant Research. 51: 209-219.

Darodjah S. 2002. Animal Reproduction Science. Animal Husbandry Faculty, Padjadjaran University. Bandung.

Feradis MP. 2010. Animal Reproduction. Alfabeta. Bandung. 113-133.

Hardjopranjoto HS. 1995. Animal reproduction disorders. Airlangga University Press. Surabaya. 28.

Heffner LJ and Danny JS. 2005. At a Glance of Reproductive System. Second Edition. Erlangga. Jakarta. 57.

Hussin AM, NW Zaid, and SO Hussain. 2014. Compensatory Structural Adaptive Modifications of Vagina in Response to Functional Demand in Goat. Veterinary Medicine International Volume 2014. Hindawi Publishing Corporation. Article ID 789816.

Kershaw CM, M Khalid, MR McGowan, K Ingram, S Leethongdee, G Wax and RJ Scaramuzzi. 2005. The Anatomy of the Sheep Cervix and Its Influence on The Transcervical Passage of an Inseminating Pipette into The Uterine Lumen. Theriogenology. 64. Issue 5: 1225-1235.

Khadiga MG, KG Mohamed and FT Doaa. 2005. The hormonal profile during the estrous cycle and gestation in damascus goats. Small Ruminant Research. 57: 85-93.

Khalifa EI, Ahmed ME, Abdel-Gaward AM and El-Zelaky OA. 2010. The Effect of Insemination Timing on Fertilization and Embryo Gender in Zaraibi Goats. Egyptian Journal of Sheep and Goat Sciences 5(1):274-275.

Mayor P, DA Guimaraes, FL Gatius and ML Bej. 2006. First postpartum estrous and pregnancy in the female collared peccary (Tayassu tajacu) from the amazon. Theriogenology. 66, Issue 8: 2001-2007

Popalayah, Ismaya, and N Ngadiyono. 2013. Effectivity of Controlled Internal Drug Release Usedon Estrous Response and Concentration of Estrogen Hormones in Kacang and Bligon Goats. Animal Husbandry Bulletin 37(3):151-152.

Redden KD, Kennedy AD, Ingalls, JR and Gilson TL. 1993. Detection of estrous by radiotelemetnc monitoring of vaginaland ear skin temperature and pedometer measurements of activity. Journal of Dairy Science. 76: 713-721.

Rezac P, I Krivánek, and M Pöschl. 2001. Changes of vaginal and vestibular impedance in dairy goats during the estrous cycle. Small Ruminant Research 42(3):183-188.

Rodriguez-Pinon M, Gonzalez R, Tasende C, Bielli A, Genovese P and GarofaloEG. 2014. Cervical Changes in Estrogen Receptor Alpha, Oxytocin Receptor, LH Receptor, and Cyclooxygenase-2 Depending on The Histologic Compartment, Longitudinal Axis, and Day of The Ovine Estrous Cycle. Theriogenology 81. Issue 6: 813–824.

Toelihere MR. 1993. Livestock Artificial Insemination. Angkasa, Bandung.

Vidal BR, Silva GFD, Santos JS, Dias FEF, Lima AKF, Viana EB,Neves WC, Viana GEN, Gomes MGT and Cavalcante TV. 2013. Estrous Identification Through Colpocytology in Sows in Intensive FreeRange Breeding. Journal of Veterinanry Advances 3(10):281-284.

Vivanco HW. 1986. Recent Developments in Reproductive Technioues of Sheep and Goats. Small Ruminant Production in the Developing Countries. Proceedings of an Expert Consultation. Food and Agriculture Organization of the United Nations. Roma. 33.

Widiyono I, PP Putro, Sarmin, P Astuti, and CMAirin. 2011. Estradiol and Progesterone Profile in Serum, Characteristics of Vulva and Cytology of Vagina in Bligon Goat during Estrous Cycle . Journal of Veterinary 12(4): 263-268.

Zaid NW. 2011. Metachromasia in the exfoliated vaginal cells of awassi ewes. Kufa Journal for Veterinary Medical Sciences 2(1): 114-120

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