FM Suhartati, Wardhana Suryapratama

Abstract


A study has been conducted to examine the effect of Mg and Co supplementation on rumen fermentation products. The study was conducted in an in vitro experiment, applying completely randomized design, 3x3 factorial. The first factor was three levels of Mg (0; 7.5 and 15.0 mM), and three levels of Co (0; 0.03 and 0.06 mM) as the second factor, total 9 treatments each repeated three times, comprising 27 experimental units all together. In vitro incubation lasted for 4 hours. Variables measured were the concentrations of VFA, N-NH3 and protein synthesis of microbial rumen. Data were subject to analysis of variance and orthogonal polynomials test. The results showed an interaction effect between Mg and Co on the concentration of VFA, N-NH3 and protein synthesis of microbial rumen. The increasing supplementation of Mg at 0.06 mM Co increased VFA concentration; the highest concentration of N-NH3 was achieved by rumen fluid supplemented with 15.0 mM of Mg and 0.03 mM of Co. The highest protein synthesis of microbial rumen was achieved by the rumen fluid supplemented with 8.18 mM of Mg with no supplementation of Co.


Full Text:

PDF

References


Baldwin RL and MJ Allison. 1983. Rumen metabolism. Journal Animal Science. 57 Suppl. 2: 461-477.

Dwipartha PS, IN Suarsana NK Suwiti. 2014. The Profiles of Mineral Potassium (K) and Cobalt (Co) in Bali Cattle Serum Raised In the Plantation Land. Buletin Veteriner Udayana Vol. 6 No. 2:125-128. In Indonesia.

General Laboratory Procedures. 1966. Departement of Dairy Scienc, University of Wicosin, USA

Ginting SP. 2005. Synchronization between Protein And Energy Degradation In Rumen to Maximize Microbial Protein Production. WARTAZOA Vol. 15 No. 1:1-10. In Indonesia.

Gyamfi ET, IK Kwarteng, MO Ansah, AK Anim, M Ackah, L Kpattah and NO Bentil. 2011. Ghana Atomic Energy Commission, National Nuclear Research Institute, Nuclear Chemistry and Environmental Research. Proceedings of the International Academy of Ecology and Environmental Sciences, 1 (3-4):179-185

Hindratiningrum N, M Bata dan SA Santosa. 2011. Rumen Fermentation Product and Microbial Protein Production of Local Cattle Fed with Ammoniated Straw and Some Feed Ingredients of Energy Source. Agripet : Vol (11) No. 2: 29-34. In Indonesia.

Khampa S and M Wanapat. 2006. Supplementation Levels of Concentrate Containing High Levels of Cassava Chip on Rumen Ecology and Microbial Protein Synthesis in Cattle. Pakistan Journal of Nutrition 5(6):501-505.

Orskov ER. 1988. Protein Nutrition in Ruminants. Second Edition. Academic Press Inc., San Diego.

Ozturk H, M Pekcan, M Sireli and UR Fidanci. 2010. Effects of propolis on in vitro rumen microbial fermentation. Ankara Üniv Vet Fak Derg, 57: 217-221

Paengkoum P, JB Liang, ZA Jelan and M Basery. 2006. Utilization of Steam-treated Oil Palm Fronds in Growing Saanen Goats: II. Supplementation with Energy and Urea. Asian-Aust. J. Anim. Sci. 19 (11): 1623-1631.

Steel RGD and JH Torrie. 1993. Prinsip dan Prosedur Statistika. Suatu Pendekatan Biometrik. Edisi kedua. Diterjemahkan oleh Sumantri, B. PT Gramedia Pustaka Utama, Jakarta.

Suwandyastuti SNO dan EA Rimbawanto. 2011. The Utilization of Fibrous Waste In Concentrate for Yearling Bull. Agripet : Vol (11) No. 1: 1-4. In Indonesia.

Suryapratama W. 1999. Efek The Supplementation

effect of branched-volatile fatty acids, Lysine Capsules and Threonine on Protein Nutrient of Holstein Cows. (Dissertation, unpublished). Program Pasca Sarjana Institut Pertanian Bogor. (unpublished). In Indonesia.

Thalib A, B Haryanto, S Kompiang, IW Mathius and A Aini. 2000. Effect Of Trace Minerals And Phenylpropionic Acid On Performances Of Coccus And Rod-Shaped Cellulolytic Bacteria In Digesting Forage Fibre. Jurnal Ilmu Ternak dan Veteriner.5(2): 92-99. In Indonesia.

Tilley JMA and RA Terry. 1963. A Two Stage Technique for the in vitro of forage crops. Jurnal of the British Gressland Society. 18(2):104.

Uhi HT. 2005. Gelatin Sago-BasedCatalitic Supplements, NPN And Trace Minerals For Ruminants In Marginal Land. [Unpublished, dissertation]. Bogor: Program Pascasarjana, Institut Pertanian Bogor.

Widyobroto BP, SPS Budi and A Agus. 2007. Effect Of Undegraded Protein And Energy Levels On Rumen Fermentation Kinetics And Microbial Protein Synthesis In Cattle. J Indonesia Trop Anim Agric. 32:194-200. In Indonesia.

Zadeh JB, Z Moradi and N Moradi. 2013. Synchronization of Energy and Protein on Supply Synthesis Microbial Protein. International Journal of Advanced Biological and Biomedical Research. 1 (6) : 594-600.

Zinn RA And FV Owens. 1995. A rapid procedure purine measuement and its use for estimating net ruminant protein synthesis. Can. J. Anim. Sci. 66:157-166.




DOI: http://dx.doi.org/10.20884/1.anprod.2016.18.2.552

Article Metrics

##”article.abstract”## view : 1442 times
PDF – 720 times

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Powered by OJS | Design by ThemeOJS