The aim of this study was to determine the levels of free fatty acids and carboxymethylcellulase activity (cmc-ase) activity of some protected fat-proteins base on in vitro Tilley and Terry method. Two sources of fat, i.e. crude palm oil and fish oil and three sources of protein i.e. skim milk, soybean flour and soybean meal were used in the formulation of protected fat-protein, and thus there were six treatment combinations. The filtrate from the in vitro test was analyzed for the levels of free fatty acids and cmcase activity. The result of this research indicates that different combinations of feed materials and fat give different content of free fatty acid in first stage and second stage in vitro, with the best results in the combination treatment of skim milk and palm oil that give the lowest result of free fatty acid concentration in fisrt stage in vitro (0.168%) and the highest result free fatty acid concentration in second stage in vitro ( 4.312%) . The activity of CMC-ase was not influenced by different sources of fat and protein. It can be concluded was that the protection of the combination between skim milk and CPO gives the highest protection results.
Ashes, JR, E Fleck and TW Scott. 1995. Dietary manipulation of membran lipids and its implications for their role in the production of second messengers. In: Ruminant Physiology: Digestion, Metabolism, Growth and Reproduction. Edited by W. v. Engelhardt, S. Leonhard-Marek, G. Breves and D. Giesecke. Ferdinand Enke Verlag. Stuttgart.
Bayourthe C, M Vernay and R Moncoulon. 1994. Effect of calsium salts of fatty acids on rumen function and the digestibility of ration by sheep. J. Sci.Food. Agric. 64: 341-347.
Bayourthe C, R Moncoulon and M Vernay. 1993. Effect of protein –protected fat on ruminal and total nutrient digestibility of sheep diets. J Anim. Sci 71:1026-1031
Cortes C, DC da Silva-Kazama, R Kazama, N Gagnon, C Benchaar, GTD Santos, LM Zeoula and HV Petit. 2010. Milk composition, milk fatty acid profile, digestion, and ruminal fermentation in dairy cows fed whole flaxseed and calcium salts of flaxseed oil. J. Dairy Sci. 93:3146-3157.
Doreau M and Y Chilliard. 1996. Digestion and metabolism of dietary fat in farm animal. In: An International Conference on Fats in The Diets of Animal and Man. Birmingham.
Gulati SK, MR Garg and TW Scott. 2005. Rumen protected protein and fat produced from oilseeds and/or meals by formaldehyde treatment; their role in ruminant production and product quality: a review. Australian Journal of Experimental Agriculture. 45:1189-1203.
Gurr MI. 1984. The chemistry and biochemistry of plant fats and their nutritional importance. In: Fats in Animal Nutrition. Edited by J. Wiseman. Butterworths. London.
Haddad SG and HM Younis. 2004. The effect of adding ruminally protected fat in fattening diets on nutrient intake, digestibility and growth performance of Awassi lamb. Anim. Feed Sci. Tech. 113:61-69.
Halliwel G, MNBA Wahab, AH Patel. 1985. Chemical composition of endo-1,4-ß-D-glucanases to cellulotic in Trichoderma koningii. J. Appl. Biochem. 7:43-45.
Hartati L. 2014. Upaya Peningkatan Asam Lemak Susu Sapi Perah Dengan Suplementasi Lemak Terproteksi. Dissertation. Gadjah Mada University. Yogyakarta.
Hartati L, A Agus, BP Widyobroto and LM Yusiati. 2012a. In Vitro Digestibilities of Six Rumen Protected Fat-Protein Supplement Formulas. Animal Production 14 (1):1-5.
Hartati L, A Agus, LM Yusiati and BP Widyobroto. 2012b. Inclusions of Rumen Protected Protein-Fat Supplements in the Ration of Lactating Dairy Cow: Effects on Feed Intake and Digestibility, Milk Production and Composition, and Milk Fatty Acid Profile. Proceeding the 15th AAAP animal Science Congress “Improving Smallholder and Industrial Livestock Production for Enhancing Food Security, Environment and Human Welfare” , 26-30 Nopember, Bangkok, Thailand.
Kiernan JA. 2000. Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: What they are and what they do. Microscopy Today 00-1:8-12.
Palmquist DL, MR Weisbjerg and T Hvelplund. 1993. Ruminal, intestinal and total digestibilities of nutrients in cows fed diets high in fat and undegradable protein. J. Dairy Sci. 76: 1353-1364.
Plummer DT. 1971. An Introduction to Practical Biochemistry. Tata McGraw-Hill Publishing Company Ltd. UK.
Purwadaria T, PA Marbun, AP Sinurat dan PP Ketaren. 2003. Perbandingan Aktivitas Enzim Selulase dari Bakteri dan Kapang Hasil Isolasi dari Rayap. JITV Vol. 8 No. 4:213-219.
Saify ZS, S Akhtar, KM Khan, S Perveen, SAM Ayattollahi, S Hassan, M Arif, SM Haider, F Ahmad, S Siddiqui and MZ Khan. 2003. A Study on the fatty acid composition of fish liver oil from two marine fish, Euspyra blochii and Carcharhinus bleekeri. Turkey J. Chemistry. 27:251-258.
Sklan D and M Tinsky, 1993. Production and reproduction response by dairy cows fed varying undegradable protein coated with rumen bypass fat. J. Dairy Sci. 76:216-223.
Sklan D. 1989. In vitro and in vivo protection of protein coated with calcium soaps of long fatty acid in ruminants. J. Agric. Sci. (Camb) 112:79-86.
Sudarmadji S, B Haryono dan Suhardi. 1984. Prosedur Analisis untuk Bahan Makanan dan Pertanian. 3thEdition. Liberty. Yogyakarta.
Tilley JMA and R A Terry. 1963. A two stage technique for in vitro digestion of forage crop. Journal of the British Grassland Society 18: 104-111.
Wettstein HR, MRL Scheeder, F Sutter and M Kreuzer. 2001. Effect of lecithins partly replacing rumen-protected fat on fatty acid digestion and composition of cow milk. Eur. J.Lipid Sci. Tecnol. 103:12-22.
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